Construct: shRNA TRCN0000262738

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001102615.1-397s21c1
DNA Barcode:
GCGTCATCTCAGGGTACAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kif19a (286942)
Gene Description:
kinesin family member 19A
Transcript:
RefSeq NM_001102615.1 (CURRENT)
Match location:
Position 397 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000262738)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTCATCTCAGGGTACAATG
Hairpin Sequence:
5'-CCGG-GCGTCATCTCAGGGTACAATG-CTCGAG-CATTGTACCCTGAGATGACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTCATCTCAGGGTACAATGCTCGAGCATTGTACCCTGAGATGACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTCATCTCAGGGTACAATGCTCGAGCATTGTACCCTGAGATGACGC-3'

Other clones with same target sequence:

(none)