Construct: shRNA TRCN0000262818

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001085476.1-2538s21c1
DNA Barcode:
ACTACACTACATCGTTTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FOXD4L6 (653404)
Gene Description:
forkhead box D4 like 6
Transcript:
RefSeq NM_001085476.1 (CURRENT)
Match location:
Position 2538 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000262818)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTACACTACATCGTTTATTT
Hairpin Sequence:
5'-CCGG-ACTACACTACATCGTTTATTT-CTCGAG-AAATAAACGATGTAGTGTAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTACACTACATCGTTTATTTCTCGAGAAATAAACGATGTAGTGTAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTACACTACATCGTTTATTTCTCGAGAAATAAACGATGTAGTGTAGT-3'

Other clones with same target sequence:

(none)