Construct: shRNA TRCN0000262834

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001099686.1-3779s21c1
DNA Barcode:
TCTTCGTTCACATCCTCTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NXF2B (728343)
Gene Description:
nuclear RNA export factor 2B
Transcript:
RefSeq NM_001099686.1 (NON-CURRENT)
Match location:
Position 3779 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000262834)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTTCGTTCACATCCTCTTTG
Hairpin Sequence:
5'-CCGG-TCTTCGTTCACATCCTCTTTG-CTCGAG-CAAAGAGGATGTGAACGAAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTTCGTTCACATCCTCTTTGCTCGAGCAAAGAGGATGTGAACGAAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTTCGTTCACATCCTCTTTGCTCGAGCAAAGAGGATGTGAACGAAGA-3'

Other clones with same target sequence:

(none)