Construct: shRNA TRCN0000262888

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018650.3-2265s21c1
DNA Barcode:
CACTTCTGGTCATCCTATTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MARK1 (4139)
Gene Description:
microtubule affinity regulating kinase 1
Transcript:
RefSeq NM_018650.3 (NON-CURRENT)
Match location:
Position 2265 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000262888)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTTCTGGTCATCCTATTAA
Hairpin Sequence:
5'-CCGG-CACTTCTGGTCATCCTATTAA-CTCGAG-TTAATAGGATGACCAGAAGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTTCTGGTCATCCTATTAACTCGAGTTAATAGGATGACCAGAAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTTCTGGTCATCCTATTAACTCGAGTTAATAGGATGACCAGAAGTG-3'

Other clones with same target sequence:

(none)