Construct: shRNA TRCN0000262890

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018650.3-1405s21c1
DNA Barcode:
TTACGAGGGAAGTACCGTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MARK1 (4139)
Gene Description:
microtubule affinity regulating kinase 1
Transcript:
RefSeq NM_018650.3 (NON-CURRENT)
Match location:
Position 1405 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000262890)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTACGAGGGAAGTACCGTATT
Hairpin Sequence:
5'-CCGG-TTACGAGGGAAGTACCGTATT-CTCGAG-AATACGGTACTTCCCTCGTAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTACGAGGGAAGTACCGTATTCTCGAGAATACGGTACTTCCCTCGTAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTACGAGGGAAGTACCGTATTCTCGAGAATACGGTACTTCCCTCGTAA-3'

Other clones with same target sequence:

(none)