Construct: shRNA TRCN0000262906

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001098805.1-2365s21c1
DNA Barcode:
TCATCAGAAAGAGGATCATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANKRD20A4 (728747)
Gene Description:
ankyrin repeat domain 20 family member A4
Transcript:
RefSeq NM_001098805.1 (CURRENT)
Match location:
Position 2365 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000262906)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCATCAGAAAGAGGATCATAA
Hairpin Sequence:
5'-CCGG-TCATCAGAAAGAGGATCATAA-CTCGAG-TTATGATCCTCTTTCTGATGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCATCAGAAAGAGGATCATAACTCGAGTTATGATCCTCTTTCTGATGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCATCAGAAAGAGGATCATAACTCGAGTTATGATCCTCTTTCTGATGA-3'

Other clones with same target sequence:

(none)