Construct: shRNA TRCN0000262939

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001130693.1-2768s21c1
DNA Barcode:
TCGACAGCCATGATGTGTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gucy2d (14918)
Gene Description:
guanylate cyclase 2d
Transcript:
RefSeq NM_001130693.1 (NON-CURRENT)
Match location:
Position 2768 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000262939)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCGACAGCCATGATGTGTATA
Hairpin Sequence:
5'-CCGG-TCGACAGCCATGATGTGTATA-CTCGAG-TATACACATCATGGCTGTCGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCGACAGCCATGATGTGTATACTCGAGTATACACATCATGGCTGTCGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCGACAGCCATGATGTGTATACTCGAGTATACACATCATGGCTGTCGA-3'

Other clones with same target sequence:

(none)