Construct: shRNA TRCN0000262967

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001122678.1-2007s21c1
DNA Barcode:
AGATAGAAATGTGGACTATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm3286 (100041354)
Gene Description:
predicted gene 3286
Transcript:
RefSeq NM_001122678.1 (NON-CURRENT)
Match location:
Position 2007 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000262967)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGATAGAAATGTGGACTATTC
Hairpin Sequence:
5'-CCGG-AGATAGAAATGTGGACTATTC-CTCGAG-GAATAGTCCACATTTCTATCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGATAGAAATGTGGACTATTCCTCGAGGAATAGTCCACATTTCTATCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGATAGAAATGTGGACTATTCCTCGAGGAATAGTCCACATTTCTATCT-3'

Other clones with same target sequence:

(none)