Construct: shRNA TRCN0000262995

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001085378.1-1798s21c1
DNA Barcode:
TTCTGGGAAGTCACCTAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Myh7b (668940)
Gene Description:
myosin, heavy chain 7B, cardiac muscle, beta
Transcript:
RefSeq NM_001085378.1 (NON-CURRENT)
Match location:
Position 1798 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000262995)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCTGGGAAGTCACCTAATTT
Hairpin Sequence:
5'-CCGG-TTCTGGGAAGTCACCTAATTT-CTCGAG-AAATTAGGTGACTTCCCAGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCTGGGAAGTCACCTAATTTCTCGAGAAATTAGGTGACTTCCCAGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCTGGGAAGTCACCTAATTTCTCGAGAAATTAGGTGACTTCCCAGAA-3'

Other clones with same target sequence:

(none)