Construct: shRNA TRCN0000263076

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001105579.1-586s21c1
DNA Barcode:
AGAATGTCACCATCCAGACTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SYNDIG1L (646658)
Gene Description:
synapse differentiation inducing 1 like
Transcript:
RefSeq NM_001105579.1 (NON-CURRENT)
Match location:
Position 586 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000263076)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAATGTCACCATCCAGACTG
Hairpin Sequence:
5'-CCGG-AGAATGTCACCATCCAGACTG-CTCGAG-CAGTCTGGATGGTGACATTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAATGTCACCATCCAGACTGCTCGAGCAGTCTGGATGGTGACATTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAATGTCACCATCCAGACTGCTCGAGCAGTCTGGATGGTGACATTCT-3'

Other clones with same target sequence:

(none)