Construct: shRNA TRCN0000263104

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001098408.1-170s21c1
DNA Barcode:
ACAGCCTCCTGAAATGATTGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GAGE12C (729422)
Gene Description:
G antigen 12C
Transcript:
RefSeq NM_001098408.1 (CURRENT)
Match location:
Position 170 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000263104)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAGCCTCCTGAAATGATTGG
Hairpin Sequence:
5'-CCGG-ACAGCCTCCTGAAATGATTGG-CTCGAG-CCAATCATTTCAGGAGGCTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAGCCTCCTGAAATGATTGGCTCGAGCCAATCATTTCAGGAGGCTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAGCCTCCTGAAATGATTGGCTCGAGCCAATCATTTCAGGAGGCTGT-3'

Other clones with same target sequence:

(none)