Construct: shRNA TRCN0000263202

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001141922.1-1559s21c1
DNA Barcode:
TGAGTGGCCAAGCTCATAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bean1 (65115)
Gene Description:
brain expressed, associated with Nedd4, 1
Transcript:
RefSeq NM_001141922.1 (CURRENT)
Match location:
Position 1559 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000263202)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAGTGGCCAAGCTCATAAAT
Hairpin Sequence:
5'-CCGG-TGAGTGGCCAAGCTCATAAAT-CTCGAG-ATTTATGAGCTTGGCCACTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAGTGGCCAAGCTCATAAATCTCGAGATTTATGAGCTTGGCCACTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAGTGGCCAAGCTCATAAATCTCGAGATTTATGAGCTTGGCCACTCA-3'

Other clones with same target sequence:

(none)