Construct: shRNA TRCN0000263209

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023066.2-1006s21c1
DNA Barcode:
GACTATGATGAACCAGTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Asph (65973)
Gene Description:
aspartate-beta-hydroxylase
Transcript:
RefSeq NM_023066.2 (NON-CURRENT)
Match location:
Position 1006 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000263209)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTATGATGAACCAGTATAT
Hairpin Sequence:
5'-CCGG-GACTATGATGAACCAGTATAT-CTCGAG-ATATACTGGTTCATCATAGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTATGATGAACCAGTATATCTCGAGATATACTGGTTCATCATAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTATGATGAACCAGTATATCTCGAGATATACTGGTTCATCATAGTC-3'

Other clones with same target sequence:

(none)