Construct: shRNA TRCN0000263299

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020818.3-8065s21c1
DNA Barcode:
GGTTTAGCAATAATGGGTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UNC79 (57578)
Gene Description:
unc-79 homolog, NALCN channel complex subunit
Transcript:
RefSeq NM_020818.3 (NON-CURRENT)
Match location:
Position 8065 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000263299)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTTTAGCAATAATGGGTTTA
Hairpin Sequence:
5'-CCGG-GGTTTAGCAATAATGGGTTTA-CTCGAG-TAAACCCATTATTGCTAAACC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTTTAGCAATAATGGGTTTACTCGAGTAAACCCATTATTGCTAAACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTTTAGCAATAATGGGTTTACTCGAGTAAACCCATTATTGCTAAACC-3'

Other clones with same target sequence:

(none)