Construct: shRNA TRCN0000263403

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144882.3-2759s21c1
DNA Barcode:
TCCATATTCTGACACTATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spats2l (67198)
Gene Description:
spermatogenesis associated, serine-rich 2-like
Transcript:
RefSeq NM_144882.3 (NON-CURRENT)
Match location:
Position 2759 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000263403)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCATATTCTGACACTATATT
Hairpin Sequence:
5'-CCGG-TCCATATTCTGACACTATATT-CTCGAG-AATATAGTGTCAGAATATGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCATATTCTGACACTATATTCTCGAGAATATAGTGTCAGAATATGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCATATTCTGACACTATATTCTCGAGAATATAGTGTCAGAATATGGA-3'

Other clones with same target sequence:

(none)