Construct: shRNA TRCN0000263406

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198004.2-218s21c1
DNA Barcode:
TTTGCACCTTGCACGACATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Idnk (75731)
Gene Description:
idnK gluconokinase homolog (E. coli)
Transcript:
RefSeq NM_198004.2 (NON-CURRENT)
Match location:
Position 218 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000263406)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTTGCACCTTGCACGACATTT
Hairpin Sequence:
5'-CCGG-TTTGCACCTTGCACGACATTT-CTCGAG-AAATGTCGTGCAAGGTGCAAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTTGCACCTTGCACGACATTTCTCGAGAAATGTCGTGCAAGGTGCAAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTTGCACCTTGCACGACATTTCTCGAGAAATGTCGTGCAAGGTGCAAA-3'

Other clones with same target sequence:

(none)