Construct: shRNA TRCN0000263419

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021403.3-1316s21c1
DNA Barcode:
GTCCTCACACTACAGTGATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Srrm3 (58212)
Gene Description:
serine/arginine repetitive matrix 3
Transcript:
RefSeq NM_021403.3 (CURRENT)
Match location:
Position 1316 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000263419)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCCTCACACTACAGTGATTC
Hairpin Sequence:
5'-CCGG-GTCCTCACACTACAGTGATTC-CTCGAG-GAATCACTGTAGTGTGAGGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCCTCACACTACAGTGATTCCTCGAGGAATCACTGTAGTGTGAGGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCCTCACACTACAGTGATTCCTCGAGGAATCACTGTAGTGTGAGGAC-3'

Other clones with same target sequence:

(none)