Construct: shRNA TRCN0000263436

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026005.3-409s21c1
DNA Barcode:
CCCATGCAAGGCGTCAGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
2610301B20Rik (67157)
Gene Description:
RIKEN cDNA 2610301B20 gene
Transcript:
RefSeq NM_026005.3 (CURRENT)
Match location:
Position 409 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000263436)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATGCAAGGCGTCAGTAAA
Hairpin Sequence:
5'-CCGG-CCCATGCAAGGCGTCAGTAAA-CTCGAG-TTTACTGACGCCTTGCATGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATGCAAGGCGTCAGTAAACTCGAGTTTACTGACGCCTTGCATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATGCAAGGCGTCAGTAAACTCGAGTTTACTGACGCCTTGCATGGG-3'

Other clones with same target sequence:

(none)