Construct: shRNA TRCN0000263539

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032350.5-443s21c1
DNA Barcode:
TGGCTCCTGCTGCACATGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C7orf50 (84310)
Gene Description:
chromosome 7 open reading frame 50
Transcript:
RefSeq NM_032350.5 (CURRENT)
Match location:
Position 443 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000263539)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGCTCCTGCTGCACATGTAT
Hairpin Sequence:
5'-CCGG-TGGCTCCTGCTGCACATGTAT-CTCGAG-ATACATGTGCAGCAGGAGCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGCTCCTGCTGCACATGTATCTCGAGATACATGTGCAGCAGGAGCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGCTCCTGCTGCACATGTATCTCGAGATACATGTGCAGCAGGAGCCA-3'

Other clones with same target sequence:

(none)