Construct: shRNA TRCN0000263752

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018302.2-884s21c1
DNA Barcode:
AGGAGAAGCTGGGATTCATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C4orf19 (55286)
Gene Description:
chromosome 4 open reading frame 19
Transcript:
RefSeq NM_018302.2 (CURRENT)
Match location:
Position 884 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000263752)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGAGAAGCTGGGATTCATTG
Hairpin Sequence:
5'-CCGG-AGGAGAAGCTGGGATTCATTG-CTCGAG-CAATGAATCCCAGCTTCTCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGAGAAGCTGGGATTCATTGCTCGAGCAATGAATCCCAGCTTCTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGAGAAGCTGGGATTCATTGCTCGAGCAATGAATCCCAGCTTCTCCT-3'

Other clones with same target sequence:

(none)