Construct: shRNA TRCN0000263821

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001078172.1-868s21c1
DNA Barcode:
AGACTGTGGCTCCTAGTGATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RTL8A (26071)
Gene Description:
retrotransposon Gag like 8A
Transcript:
RefSeq NM_001078172.1 (CURRENT)
Match location:
Position 868 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000263821)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACTGTGGCTCCTAGTGATG
Hairpin Sequence:
5'-CCGG-AGACTGTGGCTCCTAGTGATG-CTCGAG-CATCACTAGGAGCCACAGTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACTGTGGCTCCTAGTGATGCTCGAGCATCACTAGGAGCCACAGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACTGTGGCTCCTAGTGATGCTCGAGCATCACTAGGAGCCACAGTCT-3'

Other clones with same target sequence:

(none)