Construct: shRNA TRCN0000263869

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016520.2-888s21c1
DNA Barcode:
GGCAACTGATGACTATCATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C9orf78 (51759)
Gene Description:
chromosome 9 open reading frame 78
Transcript:
RefSeq NM_016520.2 (NON-CURRENT)
Match location:
Position 888 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000263869)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCAACTGATGACTATCATTA
Hairpin Sequence:
5'-CCGG-GGCAACTGATGACTATCATTA-CTCGAG-TAATGATAGTCATCAGTTGCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCAACTGATGACTATCATTACTCGAGTAATGATAGTCATCAGTTGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCAACTGATGACTATCATTACTCGAGTAATGATAGTCATCAGTTGCC-3'

Other clones with same target sequence:

(none)