Construct: shRNA TRCN0000263881

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001135811.1-402s21c1
DNA Barcode:
TCTCGATTCACTGACAGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SINHCAF (58516)
Gene Description:
SIN3-HDAC complex associated factor
Transcript:
RefSeq NM_001135811.1 (CURRENT)
Match location:
Position 402 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000263881)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTCGATTCACTGACAGTAAA
Hairpin Sequence:
5'-CCGG-TCTCGATTCACTGACAGTAAA-CTCGAG-TTTACTGTCAGTGAATCGAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTCGATTCACTGACAGTAAACTCGAGTTTACTGTCAGTGAATCGAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTCGATTCACTGACAGTAAACTCGAGTTTACTGTCAGTGAATCGAGA-3'

Other clones with same target sequence:

(none)