Construct: shRNA TRCN0000263914

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025057.2-2552s21c1
DNA Barcode:
CTTCCAAGTTACCAATCATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BBOF1 (80127)
Gene Description:
basal body orientation factor 1
Transcript:
RefSeq NM_025057.2 (NON-CURRENT)
Match location:
Position 2552 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000263914)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCCAAGTTACCAATCATAT
Hairpin Sequence:
5'-CCGG-CTTCCAAGTTACCAATCATAT-CTCGAG-ATATGATTGGTAACTTGGAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCCAAGTTACCAATCATATCTCGAGATATGATTGGTAACTTGGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCCAAGTTACCAATCATATCTCGAGATATGATTGGTAACTTGGAAG-3'

Other clones with same target sequence:

(none)