Construct: shRNA TRCN0000263920

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022749.5-2704s21c1
DNA Barcode:
AGCCTTGTCTGTGTCACATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM160B2 (64760)
Gene Description:
family with sequence similarity 160 member B2
Transcript:
RefSeq NM_022749.5 (NON-CURRENT)
Match location:
Position 2704 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000263920)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCCTTGTCTGTGTCACATTA
Hairpin Sequence:
5'-CCGG-AGCCTTGTCTGTGTCACATTA-CTCGAG-TAATGTGACACAGACAAGGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCCTTGTCTGTGTCACATTACTCGAGTAATGTGACACAGACAAGGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCCTTGTCTGTGTCACATTACTCGAGTAATGTGACACAGACAAGGCT-3'

Other clones with same target sequence:

(none)