Construct: shRNA TRCN0000263921

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022749.5-1733s21c1
DNA Barcode:
CATACGTCCACGATGCTTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM160B2 (64760)
Gene Description:
family with sequence similarity 160 member B2
Transcript:
RefSeq NM_022749.5 (NON-CURRENT)
Match location:
Position 1733 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000263921)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATACGTCCACGATGCTTATG
Hairpin Sequence:
5'-CCGG-CATACGTCCACGATGCTTATG-CTCGAG-CATAAGCATCGTGGACGTATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATACGTCCACGATGCTTATGCTCGAGCATAAGCATCGTGGACGTATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATACGTCCACGATGCTTATGCTCGAGCATAAGCATCGTGGACGTATG-3'

Other clones with same target sequence:

(none)