Construct: shRNA TRCN0000263925

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_203305.2-585s21c1
DNA Barcode:
AGTCCAAGATCTCCGGCTACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM102A (399665)
Gene Description:
family with sequence similarity 102 member A
Transcript:
RefSeq NM_203305.2 (CURRENT)
Match location:
Position 585 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000263925)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTCCAAGATCTCCGGCTACA
Hairpin Sequence:
5'-CCGG-AGTCCAAGATCTCCGGCTACA-CTCGAG-TGTAGCCGGAGATCTTGGACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTCCAAGATCTCCGGCTACACTCGAGTGTAGCCGGAGATCTTGGACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTCCAAGATCTCCGGCTACACTCGAGTGTAGCCGGAGATCTTGGACT-3'

Other clones with same target sequence:

(none)