Construct: shRNA TRCN0000263947
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_138471.1-767s21c1
- DNA Barcode:
- TCTCCTTGACTCTGAGGATAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SPINDOC (144097)
- Gene Description:
- spindlin interactor and repressor of chromatin binding
- Transcript:
- RefSeq NM_138471.1 (NON-CURRENT)
- Match location:
- Position 767 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000263947)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TCTCCTTGACTCTGAGGATAA
- Hairpin Sequence:
-
5'-CCGG-TCTCCTTGACTCTGAGGATAA-CTCGAG-TTATCCTCAGAGTCAAGGAGA-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTCTCCTTGACTCTGAGGATAACTCGAGTTATCCTCAGAGTCAAGGAGATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATCTCCTTGACTCTGAGGATAACTCGAGTTATCCTCAGAGTCAAGGAGA-3'
Other clones with same target sequence:
(none)
