Construct: shRNA TRCN0000263952

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018232.1-3588s21c1
DNA Barcode:
AGTGGAAGCCAAGTCTATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM21B (55747)
Gene Description:
family with sequence similarity 21, member B
Transcript:
RefSeq NM_018232.1 (NON-CURRENT)
Match location:
Position 3588 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000263952)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTGGAAGCCAAGTCTATATT
Hairpin Sequence:
5'-CCGG-AGTGGAAGCCAAGTCTATATT-CTCGAG-AATATAGACTTGGCTTCCACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTGGAAGCCAAGTCTATATTCTCGAGAATATAGACTTGGCTTCCACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTGGAAGCCAAGTCTATATTCTCGAGAATATAGACTTGGCTTCCACT-3'

Other clones with same target sequence:

(none)