Construct: shRNA TRCN0000263975

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001010907.1-214s21c1
DNA Barcode:
ATGCATGGTATTTCACTTAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C9orf153 (389766)
Gene Description:
chromosome 9 open reading frame 153
Transcript:
RefSeq NM_001010907.1 (NON-CURRENT)
Match location:
Position 214 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000263975)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGCATGGTATTTCACTTAAC
Hairpin Sequence:
5'-CCGG-ATGCATGGTATTTCACTTAAC-CTCGAG-GTTAAGTGAAATACCATGCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGCATGGTATTTCACTTAACCTCGAGGTTAAGTGAAATACCATGCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGCATGGTATTTCACTTAACCTCGAGGTTAAGTGAAATACCATGCAT-3'

Other clones with same target sequence:

(none)