Construct: shRNA TRCN0000264066

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001080824.1-611s21c1
DNA Barcode:
CCTGACTGAAGTGGACATTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRABD2A (129293)
Gene Description:
TraB domain containing 2A
Transcript:
RefSeq NM_001080824.1 (NON-CURRENT)
Match location:
Position 611 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264066)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGACTGAAGTGGACATTAA
Hairpin Sequence:
5'-CCGG-CCTGACTGAAGTGGACATTAA-CTCGAG-TTAATGTCCACTTCAGTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGACTGAAGTGGACATTAACTCGAGTTAATGTCCACTTCAGTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGACTGAAGTGGACATTAACTCGAGTTAATGTCCACTTCAGTCAGG-3'

Other clones with same target sequence:

(none)