Construct: shRNA TRCN0000264147

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014431.2-3374s21c1
DNA Barcode:
TGTTCTGGATAGATCTATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PALD1 (27143)
Gene Description:
phosphatase domain containing paladin 1
Transcript:
RefSeq NM_014431.2 (NON-CURRENT)
Match location:
Position 3374 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264147)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTTCTGGATAGATCTATTAT
Hairpin Sequence:
5'-CCGG-TGTTCTGGATAGATCTATTAT-CTCGAG-ATAATAGATCTATCCAGAACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTTCTGGATAGATCTATTATCTCGAGATAATAGATCTATCCAGAACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTTCTGGATAGATCTATTATCTCGAGATAATAGATCTATCCAGAACA-3'

Other clones with same target sequence:

(none)