Construct: shRNA TRCN0000264166

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001012706.1-1826s21c1
DNA Barcode:
TCACGAACCGAGAGGTCTTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LINC01588 (283551)
Gene Description:
long intergenic non-protein coding RNA 1588
Transcript:
RefSeq NM_001012706.1 (NON-CURRENT)
Match location:
Position 1826 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264166)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCACGAACCGAGAGGTCTTTC
Hairpin Sequence:
5'-CCGG-TCACGAACCGAGAGGTCTTTC-CTCGAG-GAAAGACCTCTCGGTTCGTGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCACGAACCGAGAGGTCTTTCCTCGAGGAAAGACCTCTCGGTTCGTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCACGAACCGAGAGGTCTTTCCTCGAGGAAAGACCTCTCGGTTCGTGA-3'

Other clones with same target sequence:

(none)