Construct: shRNA TRCN0000264179

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021214.1-1659s21c1
DNA Barcode:
CATCAACTGTAACCATATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ABHD17C (58489)
Gene Description:
abhydrolase domain containing 17C
Transcript:
RefSeq NM_021214.1 (NON-CURRENT)
Match location:
Position 1659 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264179)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCAACTGTAACCATATAAA
Hairpin Sequence:
5'-CCGG-CATCAACTGTAACCATATAAA-CTCGAG-TTTATATGGTTACAGTTGATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCAACTGTAACCATATAAACTCGAGTTTATATGGTTACAGTTGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCAACTGTAACCATATAAACTCGAGTTTATATGGTTACAGTTGATG-3'

Other clones with same target sequence:

(none)