Construct: shRNA TRCN0000264187

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_176677.1-917s21c1
DNA Barcode:
TGCCAAGGACAACTCCATCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NHLRC4 (283948)
Gene Description:
NHL repeat containing 4
Transcript:
RefSeq NM_176677.1 (NON-CURRENT)
Match location:
Position 917 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264187)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCCAAGGACAACTCCATCAA
Hairpin Sequence:
5'-CCGG-TGCCAAGGACAACTCCATCAA-CTCGAG-TTGATGGAGTTGTCCTTGGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCCAAGGACAACTCCATCAACTCGAGTTGATGGAGTTGTCCTTGGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCCAAGGACAACTCCATCAACTCGAGTTGATGGAGTTGTCCTTGGCA-3'

Other clones with same target sequence:

(none)