Construct: shRNA TRCN0000264212

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001098621.1-640s21c1
DNA Barcode:
ACACTCCTTGAAAGCTCAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMEM251 (26175)
Gene Description:
transmembrane protein 251
Transcript:
RefSeq NM_001098621.1 (NON-CURRENT)
Match location:
Position 640 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264212)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACACTCCTTGAAAGCTCAATT
Hairpin Sequence:
5'-CCGG-ACACTCCTTGAAAGCTCAATT-CTCGAG-AATTGAGCTTTCAAGGAGTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACACTCCTTGAAAGCTCAATTCTCGAGAATTGAGCTTTCAAGGAGTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACACTCCTTGAAAGCTCAATTCTCGAGAATTGAGCTTTCAAGGAGTGT-3'

Other clones with same target sequence:

(none)