Construct: shRNA TRCN0000264261
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_183224.2-982s21c1
- DNA Barcode:
- ACGGTAACTCTCGGAGGTCAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Fam19a3 (329731)
- Gene Description:
- family with sequence similarity 19, member A3
- Transcript:
- RefSeq NM_183224.2 (NON-CURRENT)
- Match location:
- Position 982 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000264261)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- ACGGTAACTCTCGGAGGTCAT
- Hairpin Sequence:
-
5'-CCGG-ACGGTAACTCTCGGAGGTCAT-CTCGAG-ATGACCTCCGAGAGTTACCGT-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGACGGTAACTCTCGGAGGTCATCTCGAGATGACCTCCGAGAGTTACCGTTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAACGGTAACTCTCGGAGGTCATCTCGAGATGACCTCCGAGAGTTACCGT-3'
Other clones with same target sequence:
(none)
