Construct: shRNA TRCN0000264279

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028314.1-736s21c1
DNA Barcode:
TGGAACTACATCATCCATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
2700097O09Rik (72658)
Gene Description:
RIKEN cDNA 2700097O09 gene
Transcript:
RefSeq NM_028314.1 (NON-CURRENT)
Match location:
Position 736 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264279)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGAACTACATCATCCATAAT
Hairpin Sequence:
5'-CCGG-TGGAACTACATCATCCATAAT-CTCGAG-ATTATGGATGATGTAGTTCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGAACTACATCATCCATAATCTCGAGATTATGGATGATGTAGTTCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGAACTACATCATCCATAATCTCGAGATTATGGATGATGTAGTTCCA-3'

Other clones with same target sequence:

(none)