Construct: shRNA TRCN0000264301

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019836.2-1559s21c1
DNA Barcode:
TTTGCCGACACATTCTATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Noa1 (56412)
Gene Description:
nitric oxide associated 1
Transcript:
RefSeq NM_019836.2 (NON-CURRENT)
Match location:
Position 1559 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264301)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTTGCCGACACATTCTATTAT
Hairpin Sequence:
5'-CCGG-TTTGCCGACACATTCTATTAT-CTCGAG-ATAATAGAATGTGTCGGCAAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTTGCCGACACATTCTATTATCTCGAGATAATAGAATGTGTCGGCAAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTTGCCGACACATTCTATTATCTCGAGATAATAGAATGTGTCGGCAAA-3'

Other clones with same target sequence:

(none)