Construct: shRNA TRCN0000264392

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_134096.2-461s21c1
DNA Barcode:
GAAGGCTGTGACTTGTTAATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam19a5 (106014)
Gene Description:
family with sequence similarity 19, member A5
Transcript:
RefSeq NM_134096.2 (CURRENT)
Match location:
Position 461 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264392)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGGCTGTGACTTGTTAATC
Hairpin Sequence:
5'-CCGG-GAAGGCTGTGACTTGTTAATC-CTCGAG-GATTAACAAGTCACAGCCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGGCTGTGACTTGTTAATCCTCGAGGATTAACAAGTCACAGCCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGGCTGTGACTTGTTAATCCTCGAGGATTAACAAGTCACAGCCTTC-3'

Other clones with same target sequence:

(none)