Construct: shRNA TRCN0000264474

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175525.3-394s21c1
DNA Barcode:
AGAGTCAGCGAGTGGACATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ssu2 (243612)
Gene Description:
ssu-2 homolog (C. elegans)
Transcript:
RefSeq NM_175525.3 (CURRENT)
Match location:
Position 394 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264474)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGTCAGCGAGTGGACATTT
Hairpin Sequence:
5'-CCGG-AGAGTCAGCGAGTGGACATTT-CTCGAG-AAATGTCCACTCGCTGACTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGTCAGCGAGTGGACATTTCTCGAGAAATGTCCACTCGCTGACTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGTCAGCGAGTGGACATTTCTCGAGAAATGTCCACTCGCTGACTCT-3'

Other clones with same target sequence:

(none)