Construct: shRNA TRCN0000264481

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145412.3-208s21c1
DNA Barcode:
GCTCGCTACAGCACCGGATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Chtf8 (214987)
Gene Description:
CTF8, chromosome transmission fidelity factor 8
Transcript:
RefSeq NM_145412.3 (CURRENT)
Match location:
Position 208 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264481)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCGCTACAGCACCGGATTA
Hairpin Sequence:
5'-CCGG-GCTCGCTACAGCACCGGATTA-CTCGAG-TAATCCGGTGCTGTAGCGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCGCTACAGCACCGGATTACTCGAGTAATCCGGTGCTGTAGCGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCGCTACAGCACCGGATTACTCGAGTAATCCGGTGCTGTAGCGAGC-3'

Other clones with same target sequence:

(none)