Construct: shRNA TRCN0000264540

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081154.1-5405s21c1
DNA Barcode:
CCATTTGAACATGGAATTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Marf1 (223989)
Gene Description:
meiosis arrest female 1
Transcript:
RefSeq NM_001081154.1 (NON-CURRENT)
Match location:
Position 5405 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264540)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATTTGAACATGGAATTAAA
Hairpin Sequence:
5'-CCGG-CCATTTGAACATGGAATTAAA-CTCGAG-TTTAATTCCATGTTCAAATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATTTGAACATGGAATTAAACTCGAGTTTAATTCCATGTTCAAATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATTTGAACATGGAATTAAACTCGAGTTTAATTCCATGTTCAAATGG-3'

Other clones with same target sequence:

(none)