Construct: shRNA TRCN0000264567

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024185.4-1450s21c1
DNA Barcode:
GACAGACGACACTCCTATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mindy3 (66960)
Gene Description:
MINDY lysine 48 deubiquitinase 3
Transcript:
RefSeq NM_024185.4 (CURRENT)
Match location:
Position 1450 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264567)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACAGACGACACTCCTATTAA
Hairpin Sequence:
5'-CCGG-GACAGACGACACTCCTATTAA-CTCGAG-TTAATAGGAGTGTCGTCTGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACAGACGACACTCCTATTAACTCGAGTTAATAGGAGTGTCGTCTGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACAGACGACACTCCTATTAACTCGAGTTAATAGGAGTGTCGTCTGTC-3'

Other clones with same target sequence:

(none)