Construct: shRNA TRCN0000264587

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029247.3-813s21c1
DNA Barcode:
GCAACTCTTCAGCTAACTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
4930550C14Rik (75311)
Gene Description:
RIKEN cDNA 4930550C14 gene
Transcript:
RefSeq NM_029247.3 (CURRENT)
Match location:
Position 813 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264587)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAACTCTTCAGCTAACTTAA
Hairpin Sequence:
5'-CCGG-GCAACTCTTCAGCTAACTTAA-CTCGAG-TTAAGTTAGCTGAAGAGTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAACTCTTCAGCTAACTTAACTCGAGTTAAGTTAGCTGAAGAGTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAACTCTTCAGCTAACTTAACTCGAGTTAAGTTAGCTGAAGAGTTGC-3'

Other clones with same target sequence:

(none)