Construct: shRNA TRCN0000264626

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028157.2-2112s21c1
DNA Barcode:
AGACACCGTCCAGGGATAAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spata31d1a (72219)
Gene Description:
spermatogenesis associated 31 subfamily D, member 1A
Transcript:
RefSeq NM_028157.2 (CURRENT)
Match location:
Position 2112 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264626)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACACCGTCCAGGGATAAAC
Hairpin Sequence:
5'-CCGG-AGACACCGTCCAGGGATAAAC-CTCGAG-GTTTATCCCTGGACGGTGTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACACCGTCCAGGGATAAACCTCGAGGTTTATCCCTGGACGGTGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACACCGTCCAGGGATAAACCTCGAGGTTTATCCCTGGACGGTGTCT-3'

Other clones with same target sequence:

(none)