Construct: shRNA TRCN0000264699

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172884.2-734s21c1
DNA Barcode:
AGTCACACAGCACGTGGATGT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
2900026A02Rik (243219)
Gene Description:
RIKEN cDNA 2900026A02 gene
Transcript:
RefSeq NM_172884.2 (NON-CURRENT)
Match location:
Position 734 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264699)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTCACACAGCACGTGGATGT
Hairpin Sequence:
5'-CCGG-AGTCACACAGCACGTGGATGT-CTCGAG-ACATCCACGTGCTGTGTGACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTCACACAGCACGTGGATGTCTCGAGACATCCACGTGCTGTGTGACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTCACACAGCACGTGGATGTCTCGAGACATCCACGTGCTGTGTGACT-3'

Other clones with same target sequence:

(none)