Construct: shRNA TRCN0000264718

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177203.1-988s21c1
DNA Barcode:
GTGGAATCTTTAAACGTATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccdc169 (320604)
Gene Description:
coiled-coil domain containing 169
Transcript:
RefSeq NM_177203.1 (NON-CURRENT)
Match location:
Position 988 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264718)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGAATCTTTAAACGTATTA
Hairpin Sequence:
5'-CCGG-GTGGAATCTTTAAACGTATTA-CTCGAG-TAATACGTTTAAAGATTCCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGAATCTTTAAACGTATTACTCGAGTAATACGTTTAAAGATTCCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGAATCTTTAAACGTATTACTCGAGTAATACGTTTAAAGATTCCAC-3'

Other clones with same target sequence:

(none)