Construct: shRNA TRCN0000264720

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177203.1-1116s21c1
DNA Barcode:
CCGTTCCTACATCGCGGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccdc169 (320604)
Gene Description:
coiled-coil domain containing 169
Transcript:
RefSeq NM_177203.1 (NON-CURRENT)
Match location:
Position 1116 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264720)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTTCCTACATCGCGGAAAT
Hairpin Sequence:
5'-CCGG-CCGTTCCTACATCGCGGAAAT-CTCGAG-ATTTCCGCGATGTAGGAACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTTCCTACATCGCGGAAATCTCGAGATTTCCGCGATGTAGGAACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTTCCTACATCGCGGAAATCTCGAGATTTCCGCGATGTAGGAACGG-3'

Other clones with same target sequence:

(none)