Construct: shRNA TRCN0000264760

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175287.3-264s21c1
DNA Barcode:
CACCTTGTGAAGGCAGAATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
A430005L14Rik (97159)
Gene Description:
RIKEN cDNA A430005L14 gene
Transcript:
RefSeq NM_175287.3 (NON-CURRENT)
Match location:
Position 264 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264760)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCTTGTGAAGGCAGAATTA
Hairpin Sequence:
5'-CCGG-CACCTTGTGAAGGCAGAATTA-CTCGAG-TAATTCTGCCTTCACAAGGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCTTGTGAAGGCAGAATTACTCGAGTAATTCTGCCTTCACAAGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCTTGTGAAGGCAGAATTACTCGAGTAATTCTGCCTTCACAAGGTG-3'

Other clones with same target sequence:

(none)